|
|
|
Closely resembling those of its well-characterized cousin, Xenopus laevis, X. tropicalis embryos are readily manipulated by techniques ranging from explant assays to transgenesis, and can be evaluated with most X. laevis molecular probes. Unlike the slow-growing, tetraploid X. laevis, however, X. tropicalis is diploid and has a relatively short (<5 months) life cycle, greatly increasing the feasibility of multigeneration genetic analysis. Our group, which includes collaborators across the United States and Europe, is continuing to develop X. tropicalis as a model organism. We are optimizing husbandry regimes to further shorten generation time, adapting X. laevis protocols, and assembling a set of genetic research tools including chemical and insertional mutagenesis, gene traps, stable transgenic reporter lines, inducible gene expression systems, and genetic and physical maps of the X. tropicalis genome. X. tropicalis Consortium |
|
This work is supported by NIH grant RR13221 |
||||
| Principal
Investigator: Robert Grainger, University of Virginia Department of Biology, Gilmer Hall Home Page PO Box 400328 Charlottesville, VA. 22904-4328 Phone: 434-982-5495 Fax: 434-982-5626 E-mail: rmg9p@virginia.edu |
|
Co-Investigators:
Enrique Amaya, University of Cambridge, England Doug DeSimone, University of Virginia Richard Harland, University of California at Berkeley Ray Keller, University of Virginia Research Associates:
Martin Offield, University of Virginia |
||