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DOROTHY SCHAFER |
| Associate Professor of Biology | |
| Email: das9w@virginia.edu | |
| Office: (434) 243-5297 | |
| Lab: (434) 243-5302 | |
| Office: 059 Gilmer Hall | |
| EDUCATION | |
| B.S., SUNY College of Environmental Science & Forestry, 1977 | |
| Ph.D., University of Michigan, 1983 | |
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| RESEARCH INTERESTS | ||||
Our research strives to understand how cells maintain their dynamic actin filament cytoskeleton required for many cellular processes, including, cell migration, maintaining shape, and for trafficking. To learn the molecular mechanisms by which dynamic actin filaments provide these diverse cellular functions, we visualize actin dynamics in living cells and complement the in vivo observations with biochemical approaches. The GTPase dynamin2 plays an unexpected role in regulating actin filament dynamics during cell migration and endocytosis. Our recent work implicates dynamin2, together with its interacting partner cortactin, as regulators of actin filament crosslinking that influences the mechanical properties of the cytoskeletal network. |
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Dynamic actin is also associated with early endosomes and appears to be essential for efficient receptor traffic via endosomal recycling pathways. We aim to understand how of dynamic endosome-associated actin facilitates recycling traffic. A long-standing interest is to know how cells move. One focus has been on capping protein, a ubiquitous actin-binding protein that regulates filament growth by "capping" the fast-growing end of actin filaments. Proteins of the Ena/VASP family antagonize capping in vitro . We have used TIRF microscopy to determine the mechanisms whereby Ena/VASP proteins regulate cell motility by antagonizing actin filament capping. |
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| Actin filaments (red) recruited to lipid (yellow) is organized in loose bundles by dynamin2 GTPase | ||||
| REPRESENTATIVE PUBLICATIONS | ||||
Schafer, D.A. , Weed, S.A., Binns, D., Karginov, A.V., Parsons, J.T. and Cooper, J.A. (2002) Dynamin2 and cortactin regulate actin assembly and filament organization. Curr. Biol. 12:1852-1857 |
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Barzik, M., Kotova, T.I., Higgs, H.N., Larnele Hazelwood, Dorit Hanein, Gertler, F.B. and Schafer, D.A. (2005) Ena/VASP proteins enhance actin polymerization in the presence of barbed end capping proteins. J. Biol. Chem. 280:28653-28662. |
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Cai, L., Marshal, T.W., Uetrecht, A.C., Schafer, D.A. and Bear, J.E. (2007) Coronin 1B coordinates Arp2/3 complex and Cofilin activities at the leading edge. Cell 128:915-929. |
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Pasic, L., Kotova, T.I., and Schafer, D.A. (2008) Ena/VASP proteins capture actin filament barbed ends. J. Biol. Chem. 283:9814 |
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© Copyright 2008-10
